| Name | Phone | Category |
| L & K Nutritional Beverages | (515) 720-6790 | Health Care |
| LaBounty Family Chiropractic | (515) 965-8280 | Health Care |
| Lakeside Center | (515) 963-3570 | Facilities |
| Larry Zach Wildlife Art | (515) 964-7472 | Retail-Shops-Stores |
| LaRue Coffee | (800) 658-4498 | Food-Beverage |
| The Lauridsen Group, Inc. | (515) 289-7600 | Agribusiness |
| Lawn Doctor of Ankeny - Altoona - Marshalltown | (515) 975-1812 | Lawn Maintenance |
| Lawyer Services, Inc. | (515) 250-2645 | Legal Services |
| Lazer Electric, LLC | (515) 263-0352 | Contractor |
| Leaning Tower of Pizza | (515) 964-0401 | Restaurants |
| The Learning Post of Ankeny | (515) 964-3494 | Retail-Shops-Stores |
| LearningRx | 515-224-4819 | Education |
| Legs On Lease Inc. | (515) 965-7578 | Personal Assistance |
| Lemar Industries Corp. | (515) 266-7264 | Manufacturing |
| Lenning Construction | (515) 290-4030 | Contractor |
| Leydens Cellular | (515) 964-5170 | Communications |
| Liberty Bank | (515) 473-7033 | Finance |
| Lifeworks Counseling and Education Services | (515) 963-3459 | |
| Lighting Solutions | (515) 965-8773 | Distributors |
| Lily Nails | (515) 963-0597 | Health & Beauty |
| Lincicum and Associates, P.C. | (515) 964-2225 | Finance |
| Little Endeavors Child Development Center | (515) 963-0500 | Day Care |
| Lorenz & Jones Marine Distributors, Inc. | (515) 964-4205 | Distributors |
| Lovely Nails | (515) 965-1292 | Health & Beauty |
| Luke Brothers Floor Covering | (515) 964-4075 | Retail-Shops-Stores |